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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Sun Mar 10, 2019 4:47 pm 
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I was able to coax one more picture out of the old battery.
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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Sun Mar 10, 2019 7:04 pm 
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Looks good. You'll have baby plants in no time.

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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Wed Mar 13, 2019 11:07 am 
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One more, now that I have a battery for my better camera...

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Quick update on the stems. Most are doing good, but growth has slowed, and still no signs of roots. I'm wondering if they aren't getting enough gas exchange to thrive. One flask contaminated. Not sure how, (probably the lids) but I'm attempting a decontamination of it using H2O2 3%. I have in the past been successful in decontaminating stem flasks using H2O2 and giving it a good flush, but I have also lost a few stems as well... Anyways this has hastened my looking into proper flasks vs reused pickle jars... If I'm going to get serious about propagation, I really shouldn't cheap out too much...

BTW: When I've been posting Photos, I've been resizing them down so they fit on the screen decently, at least for my screen, are they fitting yours good or do I need to make them slightly smaller? 800w used to be the goal, but I recently up'd to 1000w, and want make sure they aren't too big for other people.


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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Wed Mar 13, 2019 2:46 pm 
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NHguy03276 wrote:
and still no signs of roots. I'm wondering if they aren't getting enough gas exchange to thrive.

Just be patient. They take awhile to acclimate to the change and then they will start growing again. They are probably getting enough gas. Dean used to use unvented jars and never had any trouble.


NHguy03276 wrote:
If I'm going to get serious about propagation, I really shouldn't cheap out too much...

I think there are cheaper options, but maybe not pickle jars. :-)
I used mason jars for years. They are relatively cheap and commonly available. You get the lids at Phytotech.

NHguy03276 wrote:
BTW: When I've been posting Photos, I've been resizing them down so they fit on the screen decently, at least for my screen, are they fitting yours good or do I need to make them slightly smaller? 800w used to be the goal, but I recently up'd to 1000w, and want make sure they aren't too big for other people.

I think 800 is probably fine for most things, but 1000 was probably best for the pic of the germinated seed.

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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Thu Mar 14, 2019 6:13 am 
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As far as remedying flask contamination, while I've never done it myself, I have has a a few people tell me that putting a bit of Concentric Ag Garden Solution in the flask does the trick. The live microorganisms kill the pathogens and by "infecting" the protocorms and seedlings, "pump" nutrients into them, making them grow faster.

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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Thu Mar 14, 2019 11:36 pm 
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Ben Belton wrote:
I think there are cheaper options, but maybe not pickle jars. :-)
I used mason jars for years. They are relatively cheap and commonly available. You get the lids at Phytotech.



Well, Pickle Jars have one serious advantage. I pay $2.00 for the jar/lid, and it comes with all these free pickles :) ... In all honesty, the jars are great for flasking, they are about 4" across, wide mouth, and about 710ml in capacity. Even reusing the lids seems pretty solid, as long as I don't modify them. But I'm no longer trusting the modified lid/syringe filter membrane.... Any link to those mason jar lids? Phytotech has some great stuff, but if you don't know exactly what you are looking for, it can sometimes be difficult to find on their site. I wish I had know about the lids before I place my order for 10 of these https://phytotechlab.com/culture-vessel ... d-jar.html , I figured I'd try them out and see if I like them. They should be in next week.

raybark wrote:
As far as remedying flask contamination, while I've never done it myself, I have has a a few people tell me that putting a bit of Concentric Ag Garden Solution in the flask does the trick. The live microorganisms kill the pathogens and by "infecting" the protocorms and seedlings, "pump" nutrients into them, making them grow faster.


I'll definitely give it a shot (order placed).


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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Sat Mar 16, 2019 4:49 am 
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raybark wrote:
As far as remedying flask contamination, while I've never done it myself, I have has a a few people tell me that putting a bit of Concentric Ag Garden Solution in the flask does the trick. The live microorganisms kill the pathogens and by "infecting" the protocorms and seedlings, "pump" nutrients into them, making them grow faster.


Ray - Am I correct in assuming that the Solution should *not* be heated before adding to flasks, otherwise heat will kill the living organisms? If I did my own flasking, I would certainly make a habit of adding this to the finals in order to promote root growth. Back in the olden days, we added a wee bit of SuperThrive to the finals and it did promote HUGE leaf growth, but probably at the expense of root growth, on some level. Actually, our employees who did nothing but de-flask and plant in community trays preferred this, since they did not have to trim the short roots when planting the flats!

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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Sat Mar 16, 2019 3:04 pm 
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Sorry I am slow to reply, working a really early shift and not getting much sleep.

NHguy03276 wrote:
Any link to those mason jar lids?

This should be the right thing. They sell a large lid too, but mason jars with the large opening aren't very common. I'd buy the pack of 12 and make sure they fit for what you can find locally. They used to sell some that had a vent already built in.
https://phytotechlab.com/closure-round- ... 70-mm.html

I wish you lived closer. Would give you a few dozen mason jars. I'm getting too old and fat to lug them up and down stairs.

NHguy03276 wrote:
Phytotech has some great stuff, but if you don't know exactly what you are looking for, it can sometimes be difficult to find on their site.

Don't get me started on their web page.

NHguy03276 wrote:
I wish I had know about the lids before I place my order for 10 of these https://phytotechlab.com/culture-vessel ... d-jar.html , I figured I'd try them out and see if I like them. They should be in next week.

Keep them and do some stem props in baby food jars. You'll be glad you did and enjoy the extra space to let them grow a little larger before you move them. Be sure to put that sealing film around them. They do not close tight.

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 Post subject: Showing of my cloning attempt...
PostPosted: Sun Mar 17, 2019 11:19 am 
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WaltonInlet wrote:
raybark wrote:
As far as remedying flask contamination, while I've never done it myself, I have has a a few people tell me that putting a bit of Concentric Ag Garden Solution in the flask does the trick. The live microorganisms kill the pathogens and by "infecting" the protocorms and seedlings, "pump" nutrients into them, making them grow faster.


Ray - Am I correct in assuming that the Solution should *not* be heated before adding to flasks, otherwise heat will kill the living organisms? If I did my own flasking, I would certainly make a habit of adding this to the finals in order to promote root growth. Back in the olden days, we added a wee bit of SuperThrive to the finals and it did promote HUGE leaf growth, but probably at the expense of root growth, on some level. Actually, our employees who did nothing but de-flask and plant in community trays preferred this, since they did not have to trim the short roots when planting the flats!
Yes Walton, that is correct.

Actually, I'd probably add a few milliliters of a mixture of KelpMax (diluted 1:250) and Garden Solution (1:100) to each "daughter" flask.

KelpMax has far more auxins than cytokinins, so that favors root growth (although you can expect faster plant growth, too), while the microorganisms in the Garden Solution will exude antibiotics that kill pathogens, and will "infect" the seedlings with mycelia and pump nutrients directly into the plants more efficiently than the roots can do so by themselves.

I find it very interesting how the kelp, the microorganisms and fertilizer work together to support each other and the plant. I have a summary of that [url="https://firstrays.com/getting-the-most-from-your-plants/"]HERE[/url].

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 Post subject: Re: Showing of my cloning attempt...
PostPosted: Sun Mar 24, 2019 2:33 pm 
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Ok, Well, I wasn't expecting to be here so soon, but do I need to be thinking about replating? Seems like it's getting a bit crowded in this flask, and it looks like it's drying up a bit more than I expected. Would those G9 babyfood jars be big enough for a couple dozen protocorms, or should I be looking at bigger?

Ben Belton wrote:
I wish you lived closer. Would give you a few dozen mason jars.


:) I wouldn't worry about it. My Step father was a old time Yankee, and wasn't happy unless we had canned at least 4 years worth of produce each growing season. I have access to hundreds of Ball mason jars of all types and sizes. I actually use 1 pt large mouth jars for drinking glasses as they were free. The fact that Phytotech sells lids that fit make me happy.

On the topic of those G9 jars/lids, yeah, I'll definitely use them, but I'm not impressed with the lids... Also what is your thoughts on Parafilm? I have a labrat friend that swears by it, but I'm curious what others think of it.

Anyways, This is one of the flasks as of today, 3/24:
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